NanoBind
Instruction:
NanoBind consists of five sub-models:
- NanoBind-seq: predicts nanobody-antigen interaction (NAI);
- NanoBind-pro: an advanced version also for NAI prediction;
- NanoBind-site: predicts binding sites;
- NanoBind-affi: predicts binding affinity;
- NanoBind-pair: compares the binding affinities of two nanobody-antigen pairs.
Based on the input format, NanoBind offers two usage modes:
1. NanoBind (Standard Mode): integrates the first four sub-models. Users need to upload one nanobody FASTA file and one antigen FASTA file. The system will output predicted NAI, binding sites, and affinity range.
2. NanoBind-pair (Comparison Mode): includes only the NanoBind-pair model. Users should upload four FASTA files representing two nanobody-antigen pairs. The model will determine which pair has a stronger binding affinity.
Detailed requirements for input files are provided in the Readme file. Please read it carefully before using the NanoBind web service. For better understanding and testing, we also provide example input files for users to download.
Note: Due to practical limitations such as server resources, we strongly recommend not uploading more than 10 sequences at once. The online serve of NanoBind is capable of handling large datasets, but processing may take considerable time.
Source code: https://github.com/hjy23/NanoBind
Datasets: https://zenodo.org/records/xxxxxxx
Example files:
NanoBind_example.zip
